HLA B27 Test: Price, Purpose, Procedure, Range, & More

The HLA-B27 (Flow Cytometry) test is a blood test used to detect the presence of the HLA-B27 protein, which is found on the surface of white blood cells. HLA-B27 is a genetic marker associated with certain autoimmune disorders, like juvenile rheumatoid arthritis, autoimmune hepatitis, lupus erythematosus, Crohn's disease, ankylosing spondylitis, reactive arthritis, and other spondyloarthropathies. The test uses flow cytometry, a highly accurate and sensitive technique that identifies specific cell surface markers. 

The test is important for identifying the presence of the HLA-B27 antigen, which may increase the risk of autoimmune conditions. Doctors may recommend the HLA B27 test to people experiencing chronic joint pain, stiffness, or inflammation in the spine or eyes, or those with a family history of autoimmune disorders.

If you are looking for the HLA-B27 test, you can choose Redcliffe Labs. The professionals here will provide you with the best diagnostic services.

Test details in brief 

Also Known As	HLA B27 (Flow Cytometry) Test
Purpose It measures	The HLA B27 (Flow Cytometry) Test detects the presence of the HLA-B27 gene, which is associated with autoimmune conditions such as ankylosing spondylitis. This test helps diagnose the cause of chronic back pain related to such conditions.
Preparation	Do not take any over-the-counter medicine without confirming with your Doctor.
Fasting	No Fasting Required
Get Reports	Within 72 hours
Cost	₹2100

Purpose of the HLA-B27 (Flow Cytometry) Test

The key purposes of the test include:

• Diagnosis- The test helps diagnose autoimmune conditions, including ankylosing spondylitis, reactive arthritis, psoriatic arthritis, and juvenile rheumatoid arthritis.
• Identify Risk- The presence of HLA-B27 may indicate a genetic predisposition to certain chronic inflammatory diseases.
• Guided Treatment- The test helps doctors determine whether a patient is HLA-B27 positive, enabling them to personalize treatment strategies, monitor disease progression, and predict the response to therapy.
• Identify Symptoms- This is useful in evaluating chronic back pain, joint inflammation, or recurring uveitis (eye inflammation). 

What does the HLA B27 (Flow Cytometry) Test detect?

The HLA B27 (Flow Cytometry) test is designed to detect the presence or absence of the HLA-B27 antigen on the surface of WBC or white blood cells. The test detects the conditions associated with the HLA-B27 antigen, including:

• Ankylosing spondylitis (AS)
• Reactive arthritis (Reiter’s syndrome)
• Psoriatic arthritis
• Juvenile idiopathic arthritis
• Inflammatory bowel disease (IBD)-associated arthritis
• Acute anterior uveitis (eye inflammation)

This helps doctors narrow down possible causes of chronic joint pain, back pain, and inflammation in younger patients. Flow cytometry enables highly accurate and efficient detection of specific antigens on cell surfaces by using fluorescent markers to identify them.

Preparation for the HLA B27 (Flow Cytometry) Test

Here is what you should keep in mind before taking the HLA-B27 test-

• Fasting: You do not need to fast before the HLA-B27 test.
• Medications: Before the test, please inform your doctor about all medications and supplements you are taking. Some medicines may affect your test results.

Wear comfortable clothing and stay hydrated before the blood draw to make the process easier. 

The procedure of the HLA B27 (Flow Cytometry) Test

The HLA-B27 test is a simple and minimally invasive procedure. Here is the procedure for the test-

1. The trained phlebotomist will tie a band or tourniquet on the hand to make the veins more visible.
2. Next, he will clean the arm area with an antiseptic solution. 
3. Using a sterile needle, he will then collect the blood from the vein in the arm.
4. He will collect the blood into a vial or tube.
5. The sample is then sent to the laboratory for analysis, where white blood cells are separated and prepared for analysis.
6. The test reports will be available within 72 hours of sample collection. After receiving the test results, consult your doctor with your reports.

Common Illnesses Associated with the HLA-B27 Gene

The HLA-B27 gene is associated with autoimmune and inflammatory conditions. Here are the most common illnesses linked to HLA-B27:

1. Ankylosing Spondylitis (AS)- This is an inflammatory disease that affects the spine and sacroiliac joints—Over 90% of people with ankylosing spondylitis test positive for HLA-B27.
2. Reactive Arthritis (Reiter’s Syndrome)- Joint inflammation develops in response to an infection, usually in the urinary or gastrointestinal tract. It often affects the knees, ankles, and feet. 
3. Psoriatic Arthritis- An inflammatory joint condition that occurs in some people with psoriasis. HLA-B27 is associated with a subtype that affects the spine.
4. Acute Anterior Uveitis- Inflammation of the eye's middle layer affects the front part.
5. Inflammatory Bowel Disease (IBD) includes Crohn's disease and ulcerative colitis, in which joint inflammation may occur alongside bowel symptoms.

What do the HLA B27 (Flow Cytometry) Test reports indicate?

The HLA-B27 test report provides a positive or negative result on whether the HLA-B27 antigen is present in your white blood cells.

1. HLA-B27 Positive

• This means that the HLA-B27 antigen is present in your blood.
• It suggests a genetic predisposition to certain autoimmune disorders, such as:

• Ankylosing spondylitis
• Reactive arthritis
• Psoriatic arthritis
• Acute anterior uveitis
• Juvenile idiopathic arthritis

The positive result does not confirm the diagnosis. Your doctor might recommend additional tests to confirm the condition. 

2. HLA-B27 Negative

• This means the HLA-B27 antigen is not detected.
• A negative result lowers the chances of developing an HLA-B27-associated disorder, but it does not completely rule out these diseases. Some people might develop the disease without having the gene.

Always consult your medical professional to understand your test results and determine the next steps.

When Do I Need the HLA-B27 PCR Test?

Your doctor may prescribe you an HLA-B-27 test if you have the following symptoms-

• Chronic Joint Pain
• Inflammation in eyes (Acute Anterior Uveitis)
• Stiffness
• Inflammation in the neck
• Inflammation in the chest
• Lower back inflammation
• A family history of autoimmune diseases

HLA B27 (Flow Cytometry) Test price at Redcliffe Labs

The HLA-B27 test price at Redcliffe Labs is INR 2100. You can book your appointment online via the Redcliffe Labs app or call us to book your test. The professionals are available to resolve all your queries. All our labs adhere to the highest quality standards, as per international guidelines, with an experienced team of doctors, pathologists, and technicians. We follow Six Sigma processes to ensure 100% accuracy in reporting. We have fully automated machines equipped with the latest technology. We are trusted by over 50,000 doctors across India. 

Price of HLA B27 Test in Different Cities with Redcliffe Labs

City	HLA B27 Price in Different Cities
HLA B27 Test Price in Delhi	₹2400
HLA B27 Test Price in Mumbai	₹2400
HLA B27 Test Price in Pune	₹2400
HLA B27 Test Price in Bangalore	₹2400
HLA B27 Test Price in Hyderabad	₹2400
HLA B27 Test Price in Lucknow	₹2400
HLA B27 Test Price in Kolkata	₹2400

Book your HLA-B27 Test Today with Redcliffe Labs

The HLA-B27 is an important diagnostic test that helps to identify the presence of the HLA-B27 protein. This is associated with a higher risk of developing certain autoimmune and inflammatory conditions, such as ankylosing spondylitis and reactive arthritis. If you experience any abnormal symptoms, consult your doctor and consider taking the HLA-B27 test. Early diagnosis can lead to more effective treatment and better outcomes. 

FAQ

1. What is the HLA-B27 by Flowcytometry test?

The HLA-B27 flow cytometry test is a blood test used to detect the presence of the HLA-B27 antigen, a protein found on the surface of white blood cells. This test is used to diagnose or rule out certain autoimmune diseases, like ankylosing spondylitis, reactive arthritis, and juvenile rheumatoid arthritis. 

2. What is the HLA-B27 test used for?

The HLA-B27 test is used to diagnose and monitor certain autoimmune diseases, like ankylosing spondylitis and other spondyloarthritides.

3. What diseases are associated with HLA-B27 positive?

HLA-B27 positivity is associated with several autoimmune and inflammatory diseases that can affect the joints, eyes, and spine. The conditions associated with HLA-B27 positivity are ankylosing spondylitis (AS), psoriatic arthritis, and reactive arthritis.

4. What is the price of the HLA-B27 test?

The HLA-B27 test price at Redcliffe Labs is INR 2100. Book your appointment with us today.

5. Is HLA-B27 life-threatening?

HLA-B27 is not life-threatening but increases the risk of developing autoimmune conditions, such as ankylosing spondylitis, that can affect quality of life and may shorten life expectancy if not managed effectively. 

6. What is the best treatment for HLA-B27-positive individuals?

The doctor may recommend treatment based on your physical and medical condition, as well as your medical history. They might recommend a combination of medications, physical therapy, and, in some cases, surgery.

7. What is the full form of HLA?

The full form of HLA is the Human Leukocyte Antigen. 

8. What foods should be avoided with HLA-B27?

For individuals with HLA-B27, foods that can trigger inflammation and worsen symptoms must be limited or avoided. These include red meat, sugar, refined flour, fried foods, gluten, and alcohol. Additionally, processed foods, high-fat foods, and excessive sodium must be avoided.