Protein in Milk: Types, Benefits, and Nutritional Facts
Medically Reviewed By
Prof. Ashok Rattan
Written By Sheena Mehta
on Feb 4, 2025
Last Edit Made By Sheena Mehta
on Feb 4, 2025

Are you a milk drinker?
Does it still grab your attention despite the many alternatives available, like coffee and tea?
If yes, today’s topic will cater to your interest: Protein in 250 ml milk and protein in 500 ml milk.
Unlike tea and coffee, cow’s milk is a highly nutritious liquid for young mammals during their first months. The most important nutrients in milk are calcium, protein, lactose, and saturated fat.
Additionally, it helps prepare various food products, such as yogurt, cheese, cream, and butter.
Protein in 250 ml cow’s milk
Cows’s milk (Full Cream) | 8.5 g of protein |
Cows milk (toned) | 8 g of protein |
Buffalo milk (full cream) | 9-10 g protein |
Skimmed milk | 8.5-9 g protein |
Soy milk | 7-8 g protein |
Almond milk | 1-1.5 g protein |
Protein in 500 ml cow’s milk
Cows’s milk (Full Cream) | 17 g protein |
Cows milk (toned) | 16 g protein |
Buffalo milk (full cream) | 18-20 g protein |
Skimmed milk | 17-18 g protein |
Soy milk | 14-16 g protein |
Almond milk | 2-3 g protein |
Before we delve into protein content in milk, let’s look at the nutrition facts of 250 ml toned and full cream milk to understand all the vitamins and minerals you are consuming.
Nutrition Facts in Milk
Full Cream milk (250 ml) | Full Cream milk (500 ml) | |
Calories | 150 kcal | 300 kcal |
Protein | 8.5 g | 17 g |
Fat | 8 g | 16 g |
Carbohydrates | 12 g | 24 g |
Calcium | 300 mg | 600 mg |
Vitamin D | 2.5 mcg | 5 mcg |
Nutrition Content in Toned Milk (250 ml) | Nutrition Content in Toned Milk (500 ml) | |
Calories | 120 kcal | 240 kcal |
Protein | 8 g | 16 g |
Fat | 4 g | 8 g |
Carbohydrates | 12 g | 24 g |
Calcium | 300 mg | 600 mg |
Vitamin D | 2.5 mcg | 5 mcg |
Which milk quantity is better: 250 ml or 500 ml
The points below show the difference between 250 ml and 500 ml to help you decide which is better.
Choose 250 ml milk (8 g protein per serving) if:
- You want to fulfill your daily nutrition needs.
- If you want more fat intake
- If you want to consume other proteins, such as legumes, eggs, and meat.
- If you are lactose intolerant
Choose 500 ml milk (16 g protein per serving):
- If you need more calcium to improve bone health.
- If you are a child in his growing years or an athlete.
- If you need higher protein intake for muscle building or recovery.
- If you want to increase your weight healthily.
Final Verdict: 250 ml of milk protein is enough to improve general health and maintain a balanced diet. A 500 ml serving is for muscle gain, improved bone health, or high-protein needs.
2 Types of Milk Protein
Milk proteins are classified into two groups depending on their solubility in water:
- Insoluble milk proteins: The insoluble types of milk proteins are called casein and constitute about 80% of milk protein.
Additionally, casein gives milk a white appearance, making cheese, promotes smooth digestion, and may also promote lower blood pressure.
- Soluble milk proteins: The soluble type of milk protein is whey protein, which constitutes about 20% of milk protein. Whey protein contains branched-chain amino acids (BCCAs), such as leucine, isoleucine, and valine.
Additionally, whey protein helps decrease blood pressure, elevates mood during periods, and promotes quick muscle recovery after a workout.
Different Types of Casein
Since casein, an insoluble protein in milk, forms the majority (80%), let’s explore it in detail. Casein belongs to the family of phosphoproteins and is categorized into four parts:
1. Alpha-casein (α-casein): This type of casein is abundant in milk. It plays an important role in the formation of curd and cheese. Besides, it efficiently transports calcium, phosphate, and protein from the mammary gland to the neonate.
2. Beta-Casein (β-casein): The two main forms of beta-casein are A1 and A2. As per the epidemiological information, the consumption of beta-casein A1 leads is not good for the heart, causing deaths due to ischaemic heart disease. On the other hand, A2-beta casein is easier to digest.
Additionally, beta-casein is a slow-release casein protein associated with the delayed-release source of amino acids.
3. Kappa-casein (K-casein): It is a mammalian protein essential for stabilizing casein micelles in milk. Besides, this casein type helps stimulate the growth of probiotic bacteria. It prevents premature coagulation and plays a critical role in cheese-making.
4. Gamma-Casein ((γ-casein): This milk protein is formed after the breakdown of beta-casein and, thus, accounts for 3% of whole casein. It can offer physiological benefits and promote digestion and gut health.
After knowing the protein content in cow’s milk containing 250 ml (8 g) and 500 ml (16 g), let’s discover the health benefits of milk’s protein in detail.
6 Health Benefits of Protein in Milk
Milk, not a hidden secret anymore, is an excellent source of protein, providing essential amino acids that support your overall health. Some of its benefits include:
- Muscle growth and strength: Yes, you heard it right—the protein in milk aids in increasing muscle protein synthesis. Drinking milk post-exercise and resistance training leads to increased muscle hypertrophy and lean mass.
- Bone health: protein, calcium, phosphorus, and vitamin K2 are essential for optimal bone health. All these nutrients are found in milk. Hence, milk consumption is beneficial for your bones and prevents osteoporosis.
- Weight management: Drinking milk rich in protein and other nutrients can help suppress appetite and help lose weight. Branched-chain amino acids (BCAAs) enhance muscle protein synthesis and skeletal muscle mass. Additionally, it may maintain lean muscle mass, which tends to be lost during weight loss.
- Support immunity: Milk consumption is also linked to supporting immunity because milk proteins like lactoferrin and immunoglobulins contribute to a stronger immune system.
- Aids in metabolism and energy levels: The high-quality protein in milk also contains metabolism-boosting powers. Besides, it provides sustained energy throughout the day.
- Heart health: Milk proteins help regulate blood pressure and support cardiovascular health.
9 Side Effects of Protein in Milk: You May Like to Know
The presence of casein and whey protein provides essential nutrients to milk drinkers, but some individuals may also experience side effects, including
- Lactose intolerance: If lactose intolerance helps digest lactose (milk sugar), you should avoid drinking milk. It will help prevent gas, bloating, and stomach cramps.
- Milk allergy: People who have an immune response to milk proteins can experience symptoms such as hives, wheezing, and vomiting. Hence, they should avoid drinking milk, as it can cause anaphylaxis in rare cases.
- Digestive discomfort: Drinking milk rich in protein can sometimes cause digestive problems such as bloating, constipation, or diarrhea, especially if you have a sensitive stomach.
- Increased mucus production: Protein in milk can trigger mucus or phlegm due to the fluid texture.
- Kidney strain: Excessive milk consumption may strain the kidneys and increase urea production, making it harder for them to work.
- Risk of acne and skin issues: Milk protein can cause skin acne, particularly in people already prone to it. So, drinking milk could make the condition worse.
- The effect of insulin-like growth factor 1 can worsen hormonal imbalance by encouraging the production of androgen hormones like testosterone.
- Bone health concerns: High protein intake can reduce calcium from bones. Therefore, drink milk in moderation to obtain benefits.
- Health risks: Cow’s milk, which contains calcium and protein, can be high in saturated fat, raising cholesterol levels and thus increasing the risk of heart disease.
Conclusion
Undeniably, milk is a rich source of high-quality protein essential for muscle growth, bone health, and overall nutrition. Which milk quantity is better for you: 250 ml or 500 ml? You can choose the one depending on your existing health condition.
Remember, moderation is key. Consult your doctor when in doubt or concerns like lactose intolerance and how much milk you should consume to obtain benefits.
Frequently Asked Questions (FAQs)
1. How much protein is present in full cream cow’s and buffalo milk?
A full cream cow’s milk of about 250 ml contains about 8.5 g protein and 17 g in 500 ml. On the other hand, a full cream buffalo milk of about 250 ml contains about 9-10g protein and 18-20 g in 500 ml.
2. What are the two major proteins in milk?
The two major proteins in milk are casein and whey protein. About 80% of milk contains casein, while only 20% contains whey protein.